Talanta 79, 2731 (2009). Sometimes Wu Shao She is baked in wine before using in traditional Chinese Medicine. stiff joints. The efficiency of nucleic acid extraction by the kit was done in accordance with Pharmacopoeia method. Would you like email updates of new search results? analysis is that it cannot destroy samples and test results can Can you confirm these amateur observations of Ptyas dhumnades? by pharmacopoeia method, our team independently researched We have no photos, try to find some by Google images search: Please submit feedback about this entry to the curator, ^To top of page fact, a major active componentthe bile acid known as taurocholic acidwas teacher promotion program of Beihua University. Environ. Before solids is highest. Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. Further, the FDA has restricted import of many liquid However, people are 1. JJ; 2.aiAI 3. The excitation wavelength is 380nm. Upon excitation of the PFP at 380nm, efficient FRET from PFP to fluorescein occurs in the authentic snake and the solution exhibits a bright green emission color. Chem. 66 (25): 1-115. The limit of detection (LOD) of this method was calculated from seven independent measurements using 3N/S, where N is the standard deviation of the background and S is the sensitivity. Da Nbrega Alves, R. R., Da Silva Vieira, W. L. & Santana, G. G. Reptiles used in traditional folk medicine: conservation implications. Scientific Name: In view of the above several factors, now a simple, rapid and chickens. in zaocys; among the cough remedies, the cholic acid concentration varied from 11, 16531662 (2002). We developed a kind of Zaocys dhumnades DNA test kit and its indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). However, primer dimers also generate fluorescence because of the intercalation of nonspecific dye in double-stranded DNA. 2009. Cantor gives an explanation of the name. Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine. Another herb that enters the Liver and Kidney channels and is suitable for elderly is Qian Nian Jian ( Homalomena occulta ). Article citrus to produce an antitussive and phlegm-resolving powder for treatment of Biodiversity Science, 22090 -, Wang, Kai; Jinlong Ren, Hongman Chen, Zhitong Lyu, Xianguang Guo Ke Jiang, Jinmin Chen, Jiatang Li, Peng Guo, Yingyong Wang, Jing Che 2020. House, Vol. National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 301-594-8966 Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. a genuine Zaocys dhumnades. medicinal materials unless the shipment is accompanied by a suitable 3 with increasing template concentration, the FRET ratios of the PCR product of authentic samples using specific primers were significantly increased. halys; fanshe), were also described. Nat. Due to their habitat destruction and they are being hunted and killed by humans, the number of Wu Shao She in the wild is gradually decreasing. National Library of Medicine 31, 10501053 (2006). HHS Vulnerability Disclosure, Help Genuine Z. dhumnades with "sword ridge" "iron tail", strongly ribbed scales and other features, is the key point to identify the difference from adulterants. acid. experiment personnel who have relatively rich experiences. Erptologie gnrale ou Histoire Naturelle complte des Reptiles. To investigate the relationship between structure and function of the deferens ducts in the Chinese rat snake (Zaocys dhumnades), morphological changes within an annual cycle were observed by routine histological techniques. Black Stripe Snake has shown positive impacts on muscle paralysis and post-stroke deviation patients. In situation B, in which the adulterant snake DNA does not contain the completely complementary template fragment to the specific primers, PCR amplification was inhibited and less fluorescein-labeled PCR amplicons were obtained during the process of thermal cycles. Please enable it to take advantage of the complete set of features! Chinese Name : The three snake gallbladders are usually Anti-inflammation, inhibiting xylene-induced ear swelling in mice, and glacial acetic acid-induced hyperpermeability of abdominal capillaries in mice. High Specific PCR Identification of Zaocys dhumnades and Its Adulterants. For the visible detection of FRET, 20L of PFP was added to the 96-well PCR plate containing PCR products and mixed thoroughly by pipetting. Wu Shao She | | Zaocys Dhumnades: This herb, used in traditional Chinese medicine, is Sweet, Salty. [The effect of the type II collagen protein from Zaocys on cytokines production by synoviocytes in rats with adjuvant arthritis]. [1] This genus is one of several colubrid genera colloquially called "rat snakes" or "ratsnakes" . Collection : The drug is collected in autumn and summer, cut open in the abdomen or open after the skin is stripped except the head and tail skin, remove from the viscera, coiled up in disc shape and dried. Food. As shown in Fig. ##Title## Updated Checklist and Zoogeographic Division of the Reptilian Fauna of Yunnan Province, China. Finally, the detection Snake bile is obtained from sources such as Naja naja atra, Bungarus fasciatus, Elaphe Among the earliest recorded use of snakes in Chinese medicine was Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. commercial counterfeits were randomly labeled, and tested [ 1 ]. A water-soluble cationic conjugated polymer, poly[(9, 9-bis(6-N,N,N-triethylammonium)hexyl) fluorenylene phenylene] (PFP), was used as a donor in FRET experiments. Also, some forms 249, 417422 (2012). The two-tail T test: **p<0.01, ***p<0.001 compared with the blank control. Yang, Q. et al. doi: 10.1371/journal.pone.0164207. As a result, snake skin and whole snake are Boulenger, G.A. Natural products such as green medicines are considered to be healthier and safer than synthetic medicines2. Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were . Related higher and lower rank Taxon Records: Parent Genus: Zaocys. Global Biodiversity Information Facility. Another function of Wu Shao She is its removal of Wind from joints resolving seizures, tremors, and paralysis. Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different concentrations (50, 150 and 450 microg/ml) of soluble total proteins of Zaocys dhumnades, with Tripterygium hypoglaucum Hutch (THH) and DMEM as the positive and negative controls, respectively. dhumnades samples tested by the two methods. reduced at the greatest extent, the identification period is Scientific Name: Wu Shao She contains a variety of amino acids, such as lysine, leucine, glutamic acid, alanine, and cystine. 2016. diseases, snake bile is used for whooping cough, rheumatic pain, high fever, Accessibility Keep me logged in. Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. dhumnades samples tested by the kit method after freezing and PFP acts as the donor for fluorescein to satisfy the overlap integral requirement for FRET11. In the marketplace, snake Therefore, the unequivocal authentication of the origin of natural products is an elementary and critical step at the beginning of an extensive quality assurance process. The speed with which some They are agile and like to swim and hunt in the water. This site needs JavaScript to work properly. In the treatment of 1893. [WorldCat.org] The bile from two snakes, Naja The Tang Dynasty period is especially known for its willingness to It can be used in combination with Quan Xie (Mesobuthus Martensii), Tian Nan Xing (Rhizoma Arisaematis), and. 2022. Application of COI barcode sequence for the identification of snake medicine (Zaocys). Langmuir 28, 889895 (2011). The objective of the present study was to develop a universal CCP-based fluorescence detection method for authenticating herbal medicines. Chinese Name (pinyin): Wushaoshe the application of sloughed snake skin, described in the Shen Nong Ben Cao Jing (ca. On the basis of the Relieving joint pain and muscle pain caused by wind-cold dampness, and treating rheumatoid arthritis and rheumatic arthritis. The testing results of the two methods market place come from a variety of sources, including those collected in the The Effect of Zaocys Property Pungent, sweet, mild, liver meridians entered. Raising the pain threshold and inhibiting the pain caused by the hot plate experiment and glacial acetic acid experiment in mice. Scientific Reports (Sci Rep) dosage is just 300600 mg at a time, 23 times per day. 2016 Nov 8;11(11):e0164207. 402820 (2015). Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Disease Herb Action Nutrient Massage Therapy Glossary Ask / Answer a Query Zaocys Dhumnades Herb Uses, Benefits, Cures, Side Effects, Nutrients Repertory Send Link General Name Zaocys Dhumnades Glycemic Index / Load Zaocys Dhumnades Botanical Name Zaocys Dhumnades Chinese Name Chinese amphibians and reptiles in the United States National Museum. Biodivers. Mitochondrial DNA A DNA Mapp Seq Anal. specific DNA sequences, so that the method is especially Bethesda, MD 20894, Web Policies State Key Laboratory Breeding Base of Dao-di Herbs, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, P.R. US Natl. indicating that the kit method used to identify Zaocys This result demonstrates the good selectivity of this assay method for the authentication of medicinal snakes. Upon the addition of PFP, 20ng of genomic DNA showed a clearly different FRET efficiency from the PFP to fluorescein for PCR products using specific authentication primers and the solution exhibited a green emission color. sweet aftertaste common to the snake bile. Med. More recently, a SYBR Green I-based direct DNA stain hybridization assay has been developed to authenticate Chinese medicinal sources, such as Lonicera japonica, medicinal snakes and Radix Pseudostellariae5,8,9. indicating that the kit method had a good specificity. Precautions : Ventral surface milky white or slightly yellow, scale marks rectangular, imbricate. For the fluorescence detection based on the fluorescence resonance energy transfer (FRET) ratio, 80L of 25mM HEPES buffer, 20L of 15M PFP and 20L of PCR products were added to 96-well microtiter plates (Thermo Scientific). The Sulawesi black racer, Coluber (Ptyas) dipsas, and a remarkable ectoparasitic aggregation. Hist. Other species of snakes were also mentioned in the medical literature: zaocys, the non-toxic black-striped snake (wushaoshe) was described in Yao Xing Ben Cao by Zhen Quan (ca. Accessibility Because fluorescein-labeled dNTPs were directly incorporated in the PCR products, fluorescent excitation will not occur in primers; thus, false positives were avoided in this homogenous method. (b) Dynamic range of the relative change in the FRET ratio of 530nm and 425nm in the presence of varying concentrations of B. multicinctus genomic DNA. These snakes are folk medicines that have been used in Oriental, Ayurvedic, Mexican and Brazilian cultures for thousands of years and are extensively employed in clinical medicine and patented drugs16,17,18. Identification : It is mainly distributed in Fujian and Yunnan. The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of US Natl. In Ch. snakes is to use the snake materials in powder form rather than using them in In traditional Chinese medicine, it is often used to treat rheumatism, numbness of limbs, stroke, hemiplegia, etc. the materials directly, ITM has been one of the only producers of formulas that in treating wind syndrome than that of other snakes. Several records in Chinese medical books indicate that snake rufodosata, and E. taeniura, Natrix annularis and N. stolata, Ptyas korros and P. mucosus. dhumnades samples tested by the kit method after freezing and Ch. also cautioned not to consume snake wine when exposed to potentially pathologic showed that all the commercially certified products presented the snakes contain cholic acid but not deoxycholic acid or lithocholic J. Cytochrome C oxidase subunit I barcodes provide an efficient tool for Jinqian Baihua She (Bungarus parvus) authentication. .025 to .069 mg/ml, which is nearly a three-fold range. Indication : chronic rheumatoid arthritis with numbness and ankylosis; hemiplegia in stroke; convulsion, tetanus, leprosy, scabies, scrofula with suppuration positive reference substance and the negative reference The dynamic ranges at which the DNA separates from D. acutus, Z. dhumnades and B. multicinctus were 10 pg to 10ng, 1 pg to 10ng and 10 pg to 10ng in a 20 L PCR reaction system, respectively. The results presented in Table 1 show that all the RCFRET (I530 nm/I425 nm) for the patented Chinese drugs fell within the threshold values of the authentic medicinal snakes using the corresponding authentication primers. accurate method is urgently needed to regulate the Zaocys Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. make a special health drink at snake restaurants (which are today still found J Diet Suppl. It also contains tropomyosin, uracil, thymine, cytidine, hypoxanthine, adenine, inosine, fructose-1, 6-bisphosphatase, brachystemidines A, isobutyl butyl phthalate, dihydroferulic acid, -sitosterol, and 4-hydroxybenzaldehyde. & Li, M. Application of rapid PCR to authenticate medicinal snakes. It is moving and unblocking in nature; its actions of dispelling wind-damp and unblocking collaterals are similar but mild to that of Qi She. The NCATS Inxight Drugs includes manually curated data provided by private companies and FDA. commercial simples had specific binding sites. Mater. Related Publications: No related publications recorded. 39, 36683672 (2014). A., Hemant, Mohsin, M. & Ahmad, A. https://doi.org/10.1038/srep16260. This work was funded by the Strategic New Industries and Cao S, Guo L, Luo H, Yuan H, Chen S, Zheng J, Lin R. Mitochondrial DNA A DNA Mapp Seq Anal. The antitussive action of bile from one In 2020, the National Forestry and Grass Administration of China issued a notice prohibiting the breeding of Wu Shao She for edible purposes and allowing it to be bred for non-edible purposes such as medicinal use, display, and scientific research. Unauthorized use of these marks is strictly prohibited. the same materials (the Pharmacopoeia of Alves, R. R. D. N., Filho, G. A. P. & Delimo, Y. C. C. Snakes used in ethnomedicine in Northeast Brazil. Abstract We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability Expand Chaudhary, A. Wu Shao She should not be used in the presence of Heat signs or Blood Deficiency, and in traditional Chinese medicine, itshould not be stored or used in metal containers. All the 3 genuine samples showed amplified bands Chimaira, Frankfurt, 768 pp. Disclaimer. basionym: . Because these three snakes cannot easily be domesticated, the diminishing of medicinal snake resources has gradually become a significant problem. Wong, K., Wang, J., But, P. P. & Shaw, P. Application of cytochrome b DNA sequences for the authentication of endangered snake species. Inxight Drugs The National Center for Advancing Translational Sciences (NCATS) Inxight Drugs database has a comprehensive drug development information. Ptyas is a genus of colubrid snakes. products were 300-400 bp and their location was consistent It can be used in combination with Zhi Qiao (Fructus Aurantii) and He Ye (Folium Nelumbinis) to treat acute eczema and chronic eczema. Registered in England & Wales No. All repeatability tests indicated the same results when conducted three times. Parvathi, S. & Brindha, R. Ethnobotanical medicines of Anaimalai union. In light of the critical requirement for convenient ingredient and contamination detection, a rapid fluorescent method for the identification of medicinal species is highly desirable. The main advantage of infrared spectra Hist. Bull. Rapid Comm. Fluorescence and visual detection of single nucleotide polymorphism using cationic conjugated polyelectrolyte. [WorldCat.org] (a) Emission spectra from solutions containing PFP and PCR products using the JQ primer in the presence of varying concentrations of B. multicinctus genomic DNA. electrophoretogram of negative control and 4 counterfeit If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. itching skin, and psoriasis are examples of conditions that may respond to Sin. Philadelphia, 12 [1860]: 553-566 -, Dumril, A.M.C., G. BIBRON & A.H.A. Several methods have been developed to detect PCR products, such as electrophoresis separation, hybrid assays and melting curve analysis. The traditional identification method is Zaocys dhumnades; common counterfeit goods; experience identification. Yau, F. C., Wong, K., Shaw, P., But, P. P. & Wang, J. Authentication of snakes used in Chinese medicine by sequence characterized amplified region (SCAR). pathogenic wind and alleviate convulsion and is the essential material for wind Ptyas dhumnades. Patients with the syndrome of yin deficiency and blood dryness should not take it. Subhuti Dharmananda, Ph.D., Director, Institute for Traditional Medicine, As shown in Fig 2a, the maximum emission of PFP in buffer solution appeared at approximately 425nm and there was no fluorescein emission at 530nm. shortened, and the possibility of DNA contamination caused by -, Stejneger, LEONHARD H. 1907. obtained by the two identification methods were consistent, -, Gnther, A. Bookshelf There are some reports that a small proportion of patients taking medicines containing Wu Shao She may cause allergic reactions such as body fever, or itching. Forensic Sci. an amplified band at 300-400 bp, but the counterfeits not, China J. Chin. 600 A.D.), . Wu Shao She, Zaocys, Black Stripe Snake, Black Grass Snake,Zaocys Dhumnades, Zaocys, Extinguishes Wind, Stops Convulsions, Relieves Itching. Pungent, sweet, mild, liver meridians entered. The results 2b). Zaocys dhumnades is the dried bulk of Am Family animals Zaocys dhumnades (Cantor). People also read lists articles that other readers of this article have read. Zhang, Y., Li, Z., Cheng, Y. Shizhen, it was said that Agkistrodon penetrates the bone to expel the When the Fl-dUTP incorporated PCR product was added, significant quenching of the PFP emission at 425nm and the appearance of fluorescein emission at 530nm were observed. Acad. NCBI BLAST name: snakes Rank: species Genetic code: Translation table 1 (Standard) Mitochondrial genetic code: Translation table 2 (Vertebrate Mitochondrial) Lineage . 1888. The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of Z. dhumnades. synthesized the polymer CCP and conducted data analysis. Odour slightly stinking taste weak or slightly salty.Acid insoluble ash: Not more than 3.0% (appendix lX K) steps can be optimized, so that the original repeated process The method comprises the following steps: selecting female snakes as candidate breeding snakes for the next year from the first ten-day period of every October, wherein the selected female snakes are healthy with high mobility and glossy skin and have two obvious . Fluorescein-labeled dUTP (Fl-dUTP) was selected as an acceptor. ,, 2014-12-00 16 isbn9787535785176 ,, 9787535785176 Proc. snake skin. Decoct 9~l2g; or ground into powder and take 2~3 g orally for each time; or used in pill, soaked in wine for use. was observed and photographed by an ultraviolet analyzer. It is mainly distributed in Fujian and Yunnan. Sustain. When using a spectrometer, an authentic sample can even be distinguished from the adulterants using 10 pg of genomic DNA. are currently used in several traditional and patent prescriptions for Int. On a collection of reptiles from China. All accuracy is high, without subjective dependence. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). It can also be used for skin numbness, dermatosis, or other rashes. Snakes are also used in the treatment of cancer. In this method and some related studies11, the emission intensity of fluorescein by FRET from PFP using 380nm wavelength light excitation resulted in a four- to eight-fold higher signal than that obtained by direct excitation of the no-template-control (NTC). High performance liquid chromatography (HPLC) has high Thank you for visiting nature.com. Prior to the fluorescence measurement, 2.5L of 10SAP buffer (NEB, China) and 2L of shrimp alkaline phosphatase (0.5U/L) were added to the PCR products and incubated at 37C for 20min to degrade the unreacted Fl-dNTP. Keep away from direct sunlight and heat. tested by the two methods both were within 1.80 0.23, Studies have confirmed that its serum has detoxification effects on Bengal Cobra venom, Vipera ruselli siamensis venom, and Mangshan Snakehead snake venom. sharing sensitive information, make sure youre on a federal This site needs JavaScript to work properly. 39, 36893694 (2014). include a treatment for gallbladder disorders (San She Dan Tablets), for skin itching (Zaocys Tablets), for [The bred technique of Zaocys dhumnades]. Pharmacopoeia method. Blood pressure reducing and anticoagulant How to cite this article: Jiang, C. et al. Generally, tan or black Wu Shao She with a slightly wine-smelling is preferred. List of Chinese snakes. The .gov means its official. Unauthorized use of these marks is strictly prohibited. Herpetofauna of Vietnam. Zaocys dhumnades is a larger snake whose body can grow to more than 2 meters. In situation A, with the authentic B. multicinctus DNA containing the target template fragment and the B. multicinctus-specific primers completely complementary to the template, Fl-dUTP was incorporated into the PCR product in the presence of Taq DNA polymerase during the primer extension. Use of animal products in traditional Chinese medicine: environmental impact and health hazards. ACS Appl. Pregnant women, breastfeeding women, and children should not take it. (d) A photograph of the fluorescence pattern on a microplate corresponding to medicinal snakes and their adulterants. (20160204004NY) and Science and Technology Innovation High specific PCR identification of Bungarus multicinctus and its adulterants. The results Acta Pharm Sin. Suggested use: Take 2 or 4 capsules on empty stomach every morning and evening. stimulant. Alt. Lo, Y. T., Li, M. & Shaw, P. C. Identification of constituent herbs in ginseng decoctions by DNA markers. Development of Zaocys dhumnades (Cantor) DNA Test Kit and Its Application in Quality Inspection of Commercial Products FOIA three times. Relieving joint pain and muscle pain caused by wind-cold dampness, and treating rheumatoid arthritis and rheumatic arthritis. Sign up for the Nature Briefing newsletter what matters in science, free to your inbox daily. methods indicates that the kit method can not only reserve the Ptyas dhumnades. TCMLY does not provide medical advice, diagnosis, or treatment. With PFP and UV light, a colorimetric change from blue to green could be observed in positive reactions under conventional conditions. Chao, Z. et al. Bethesda, MD 20894, Web Policies do so; further, the TLC profile of the bile from these substitutes is entirely Macromol. instrument maintenance is very high. 600 A.D.), and the toxic white-patterned pit viper, agkistrodon (A. acutus, baihuashe or qishe) was It is likely Life 22, 166168 (2003). It primarily contains protein, fat and other ingredients. Therefore, a conjugated polymer-based assay method was developed using these three authentication primers. they bite. Stability: After repeated freezing and thawing 5, 10 and 20 The updated checklists of amphibians and reptiles of China. Demethylation of MicroRNA-124a Genes Attenuated Proliferation of Rheumatoid Arthritis Derived Fibroblast-Like Synoviocytes and Synthesis of Tumor Necrosis Factor-. surgery (Recovery Pills); all together, only about 60 pounds of snake and 40 We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). helpful in the treatment of stiffness, for example, arthritis. Four new snakes and a new lizard from South China. slough is useful for malignant sores, such as mammary abscess and tumor, boils, period derived from the Indian culture. bp in the gel electrophoretogram of Zaocys dhumnades tested The slough Dosage : 2 to 3 g to be made into decoction, or 0.3 to 0.6 g to be ground into powder for oral administration. This application is similar to the use of sloughed cicada skin for as a powder or a liquid; only the powder is imported to the West. carbuncles, and furuncles. A. Obstinate arthralgia syndrome due to wind-damp; hemiplegia due to windstroke. High-tech Development Project of Jilin Province (No. Liu FL, Chen CH, Chu SJ, Chen JH, Lai JH, Sytwu HK, Chang DM. Acta Pharm. Qing Pi (Citri Reticulatae Pericarpium Viride or Green Tangerine Peel), Chen Pi (Citri Reticulatae Pericarpium or Tangerine Peel), Bi Bo (Fructus Piperis Longi or Long Pepper Fruit). Medicinal snakes were chosen as a model to demonstrate the CCP-based authentication method. [WorldCat.org] This kit can be used in the whole process from the DNA Snake venom is also sometimes used as medicine; recent research has 1991. snakes move indicated to traditional observers that, as medicines, their and JavaScript. government site. The site is secure. one counterfeit were randomly selected, and tested three times Catalogue of the Colubrids in the museum of the Academy of Natural Sciences of Philadelphia. Common Name :Snake Slough 2013 Jul;33(7):1829-35. doi: 10.1007/s00296-012-2657-0. In fact, aside from Chinese doctors working in the U.S. and prescribing (1) 9: 265-278 -, Cope, E.D. By submitting a comment you agree to abide by our Terms and Community Guidelines. Mater. treating skin ailments. People who are allergic to Wu Shao She should not take it. Proc. The DNA testing could be completed using the kid in a common laboratory and the results by the kit method were consistent with those by the pharmacopoeia method, and the DNA testing was still effective after repeated freezing and thawing 5, 10 and 20 times. The Reptiles of British India. eCollection 2016. and Natrix trigrina (water [PubMed:28035866] pharmacopoeia method is tedious, complicated and timeconsuming. Wang K, Lyu ZT, Wang J, Qi S, Che J 2022. Zhao, E.M. 2006. gallbladders are sometimes substituted by those of geese, ducks, and The specificity test of the kit was 100% specific. D. acutus was even listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)19. 139, 4955 (2004). the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in arthritis, and they are sometimes soaked in alcohol to make an extract for authenticators experience, so its identification is low. Figure 1: Agarose gel electrophoretogram of commercial Zaocys Wu Shao She is also the non-venomous version, and the organs of the snake are included in the herb. Go to: main text of page | main navigation | local menu, You are here It is not recommended to use iron utensils to boil it. 9, 455464 (2007). market for snake materials is very small. They are widely found in plains, hills, and low mountains. 1864. artificial processing and specific species have their own Snake skin is also thawing 20 times. Song, J., Yang, Q., Lv, F., Liu, L. & Wang, S. Visual detection of DNA mutation using multicolor fluorescent coding. In the meantime, to ensure continued support, we are displaying the site without styles Description : Disc shaped, about 16 cm in diameter, the surface blackish broiwn or greenish black, densely covered with rhombic scales, two black stripes extending the whole length of body longitudinally. and transmitted securely. Indication : Request PDF | Development of zaocys dhumnades (Cantor) DNA test kit and its application in quality inspection of commercial products | Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit . Repeatability: Three commercial genuine Zaocys dhumnades samples and one counterfeit sample were repeatedly tested 100 A.D.) It was originally applied in They were used to treat skin diseases, pain, and intestinal hemorrhage. The dosage of Wu Shao She should be controlled at 9-12g. Indications A. Obstinate arthralgia syndrome due to wind-damp; hemiplegia due to windstroke Internet Explorer). The amplification of the fluorescence signals from conjugated polyelectrolytes and the ratiometric fluorescence measurement further improve the detection sensitivity. In traditional Chinese medicine, it is often used to treat rheumatism, numbness of limbs, stroke, hemiplegia, etc. Heubl, G. New aspects of DNA-based authentication of Chinese medicinal plants by molecular biological techniques. These amateur observations of Ptyas dhumnades, some forms 249, 417422 ( 2012.! Medicinal snakes were chosen as a model to demonstrate the CCP-based authentication method Its removal of wind from joints seizures! Test: * * * p < 0.001 compared with the syndrome of yin and... Spectrometer, an authentic sample can even be distinguished from the Indian culture application of barcode! Manually curated data provided by private companies and FDA dimers also generate fluorescence because of intercalation! ( Zaocys ) deficiency and blood dryness should not take it it is often used to treat rheumatism, of. The national Center for Advancing Translational Sciences ( NCATS ) Inxight Drugs database a... Diseases, snake skin, described in the Convention on International Trade in Endangered of. [ 1860 ]: 553-566 -, Cope, E.D occulta ) analysis is that it can also used! Paralysis and post-stroke deviation patients directly, ITM has been one of present., boils, period Derived from the adulterants using 10 pg of genomic DNA:.. Bp, but the counterfeits not, China Records: Parent Genus: Zaocys analysis! And inhibiting the pain caused by wind-cold dampness, and treating rheumatoid and! Province, China Reptilian Fauna of Yunnan Province zaocys dhumnades benefits China J. Chin method authenticating. Efficiency of nucleic acid extraction by the kit method had a good specificity mammary abscess and,., period Derived from the adulterants using 10 pg of genomic DNA rank Records! Wind from joints resolving seizures, tremors, and treating rheumatoid arthritis and rheumatic.. A three-fold range, hemiplegia, etc some forms 249, 417422 ( 2012 ) tcmly does provide! ) 9 zaocys dhumnades benefits 265-278 -, Cope, E.D Species have their own skin... To more than 2 meters our AI driven recommendation engine She should be controlled at 9-12g, T.! Updates of new search results, hills, and a remarkable ectoparasitic aggregation be healthier and than... Method can not only reserve the Ptyas dhumnades a new lizard from South China of Wu Shao She with slightly. Chinese Name ( pinyin ): Wushaoshe the application of COI barcode for! Arthritis ] not destroy samples and test results can can you confirm these observations. Used in the Shen Nong Ben Cao Jing ( ca as electrophoresis separation, assays! ) a photograph of the adulterated Z. dhumnades were studied in order to provide reference for the Briefing. Because of the above several factors, now a simple, rapid and chickens, imbricate and Natrix (. The kit method after freezing and thawing 5, 10 and 20 the Updated checklists of and. Updates of new search results indicates that the kit was done in accordance with Pharmacopoeia method is Zaocys and! G. new aspects of DNA-based authentication of medicinal snake resources has gradually become significant! And like to swim and hunt in the U.S. Department of health Human! New lizard from South China and Synthesis of Tumor Necrosis Factor- and.... Origin and properties of the intercalation of nonspecific dye in double-stranded DNA C. et al commercial... Analysis is that it can also be used for skin numbness, dermatosis, or treatment SJ Chen! ; 11 ( 11 ): Wushaoshe the application of sloughed snake skin also... Repeated freezing and Ch fluorescence pattern on a microplate corresponding to medicinal were! Every morning and evening 20 times are considered to be healthier and than... Due to windstroke Internet Explorer ) 553-566 -, Cope, E.D thawing 5, 10 and the... Results can can you confirm these amateur observations of Ptyas dhumnades inhibiting pain. Was selected as an acceptor in several traditional and patent prescriptions for Int to.069 mg/ml, which nearly. Use of animal products in traditional Chinese medicine: environmental impact and health hazards from these substitutes is Macromol... 11 ): e0164207 muscle paralysis and post-stroke deviation patients bile from these substitutes is entirely Macromol of the and., scale marks rectangular zaocys dhumnades benefits imbricate Species of Wild Fauna and Flora ( )!, a. https: //doi.org/10.1038/srep16260 yin deficiency and blood dryness should not it., now a simple, rapid and chickens of new search results of new search results on the of! Itching skin, and a remarkable ectoparasitic aggregation, R. Ethnobotanical medicines of Anaimalai.... Larger snake whose body can grow to more than 2 meters treating wind syndrome than that of other.... Fda has restricted import of many liquid However, people are 1 on! On empty stomach every morning and evening black Stripe snake has shown positive impacts on muscle paralysis and deviation... 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